mouse monoclonal antibody to cd11b Search Results


mouse anti human cd11b  (OriGene)
90
OriGene mouse anti human cd11b
Mouse Anti Human Cd11b, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+(ITGAM)+Mouse+Monoclonal+Antibody/pm26475927-54-31-51
Average 90 stars, based on 1 article reviews
mouse anti human cd11b - by Bioz Stars, 2026-10
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cd11b  (OriGene)
93
OriGene cd11b
( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs <t>(CD11b</t> + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.
Cd11b, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+(ITGAM)+Mouse+Monoclonal+Antibody/pmc12126219-266-42-44
Average 93 stars, based on 1 article reviews
cd11b - by Bioz Stars, 2026-10
93/100 stars
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anti mouse itgam cd11b  (OriGene)
90
OriGene anti mouse itgam cd11b
( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs <t>(CD11b</t> + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.
Anti Mouse Itgam Cd11b, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+(ITGAM)+Mouse+Monoclonal+Antibody/pmc06279338-295-13-17
Average 90 stars, based on 1 article reviews
anti mouse itgam cd11b - by Bioz Stars, 2026-10
90/100 stars
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mouse anti cd11b  (OriGene)
90
OriGene mouse anti cd11b
( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs <t>(CD11b</t> + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.
Mouse Anti Cd11b, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+Mouse+Monoclonal+Antibody/pmc09078433-71-31-35
Average 90 stars, based on 1 article reviews
mouse anti cd11b - by Bioz Stars, 2026-10
90/100 stars
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an ti cd11b fluorescein isothiocyanate fitc conjugated  (OriGene)
92
OriGene an ti cd11b fluorescein isothiocyanate fitc conjugated
( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs <t>(CD11b</t> + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.
An Ti Cd11b Fluorescein Isothiocyanate Fitc Conjugated, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+(ITGAM)+Mouse+Monoclonal+Antibody/10__3390_slash_su16114550-71-32-36
Average 92 stars, based on 1 article reviews
an ti cd11b fluorescein isothiocyanate fitc conjugated - by Bioz Stars, 2026-10
92/100 stars
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antibodies against cd11b  (OriGene)
90
OriGene antibodies against cd11b
( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs <t>(CD11b</t> + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.
Antibodies Against Cd11b, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+antibody+to+cd11b/CD11b+(ITGAM)+Mouse+Monoclonal+Antibody/10__1097_slash_ccm__0b013e31818441ee-98-23-26
Average 90 stars, based on 1 article reviews
antibodies against cd11b - by Bioz Stars, 2026-10
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cd11b itgam mouse monoclonal antibody  (OriGene)
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CD11b ITGAM mouse monoclonal antibody clone BEAR 1 Purified
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cd11b mouse monoclonal antibody  (OriGene)
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CD11b mouse monoclonal antibody clone OX 42 Biotin
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mouse monoclonal anti-rat cd11b/c antibody  (Angio-Proteomie)
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Image Search Results


( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs (CD11b + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.

Journal: The Journal of Clinical Investigation

Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs

doi: 10.1172/JCI181243

Figure Lengend Snippet: ( A ) Schematic diagram of the microbiota-treated Apc min/+ tumorigenesis mouse model administration method ( n = 5). ( B ) Colonization efficiency of E. coli and F. nucleatum was assessed by metagenomic sequencing analysis of Apc min/+ mice after 8 days (5 days of antibiotics treatment to deplete their gut microbiota, followed by 3 days of F. nucleatum , E . coli , or PBS orally administration for microbiota colonization) . The relative abundance of Operational Taxonomic Units (OTUs) in fecal bacterial is shown. ( C ) Representative images of tumors in the intestines of Apc min/+ mice are shown. ( D ) Statistics of tumor load of the intestines derived from Apc min/+ mice treated with PBS, E. coli , or F. nucleatum ( n = 5). ( E ) Representative images of tumorigenesis of intestines in Apc min/+ mice visualized by H&E staining. ( F ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) (n =5). ( G ) The percentages of PMN-MDSCs (CD11b + Ly6G + Ly6C low ) and M-MDSCs (CD11b + Ly6G - Ly6C hi ) in TILs (CD45 + ) of Apc min/+ mice were determined by flow cytometry sorting. ( H and I ) Statistical chart of PMN-MDSCs and M-MDSCs ( n = 5). ( J ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. ( K ) Statistical chart of Tregs ( n = 5). ( L ) Tumor-infiltrating CD8 + T cells in TILs (CD45 + ) of Apc min/+ mice, detected by flow cytometry sorting. (M). Statistical chart of CD8 + T cells ( n = 5). ( N ) The granule productions (GzmB + ) of CD8 + T cells. ( O ) Statistical chart of GzmB + cells ( n = 5). Data and error bars represent the mean ± SD of 3 independent experiments. Statistical analyses were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005.

Article Snippet: The sections were incubated overnight using primary antibodies CD8 (rabbit; Abcam, ab209775; 1:1,000), CD4 (rabbit; Bioss, bs-0647R; 1:1,000), FOXP3 (rabbit; eBioscience, 14-4771-80; 1:300), Gr-1 (rabbit; BioLegend, 108401; 1:300), CXCL3 (rabbit; Immunoway, YT2075; 1:1,000), CD8 (rabbit; Zsbio, ZA-0508), CD33 (rabbit; Abcam, ab269456), and CD11b (mouse; ORIGENE, TA807952; 1:500) at 4°C.

Techniques: Sequencing, Derivative Assay, Staining, Flow Cytometry, Comparison

( A ) Knockdown of Ccl20 and Cxcl3 inhibits the growth of CT26 subcutaneous tumors in nude mice ( n = 5). A photograph of CT26 subcutaneous tumors in nude mice and a graph of tumor growth are shown. ( B ) Knockdown of Ccl20 and Cxcl3 inhibits the growth of CT26 subcutaneous tumors in BALB/c mice ( n = 5). A photograph of CT26 subcutaneous tumors in BALB/c mice and a graph of tumor growth are shown. ( C ) Neutralizing antibodies of Ccl20 and Cxcl3 inhibited subcutaneous tumors in BALB/c mice( n = 6). A photograph of CT26 subcutaneous tumors in BALB/c mice and a graph of tumor growth are shown. ( D ) Knockdown of Ccl20 and Cxcl3 inhibits the progression of CT26 orthotopic implanted tumor in BALB/c mice ( n = 5). Representative tumor images and tumor load are shown. ( E ) The percentage of PMN-MDSCs (CD11b + Ly6G + Ly6C low ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. A bar chart indicating statistical values is presented ( n = 5). ( F ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. A bar chart indicating statistical values is presented ( n = 5). ( G and H ) Tumor-infiltrating CD8 + T cells and their granule production (GzmB + ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. Bar charts indicating statistical values are presented ( n = 5). ( I ) MDSCs (Gr-1 + ), Tregs (CD4 + , Foxp3 + ), and CD8 + T-cell infiltration in tumor tissues of orthotopic implanted CRC mice. Representative IHC images are shown. ( J ) Histogram showing the number of Gr-1 + , CD4 + , Foxp3 + , and CD8 + cells per ×20 objective lens visual field ( n = 5). Data represent the mean ± SD of 3 independent experiments. We used 2-way ANOVA to determine statistical significance of subcutaneous tumor volume. The remaining statistical methods were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005. sh, short hairpin.

Journal: The Journal of Clinical Investigation

Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs

doi: 10.1172/JCI181243

Figure Lengend Snippet: ( A ) Knockdown of Ccl20 and Cxcl3 inhibits the growth of CT26 subcutaneous tumors in nude mice ( n = 5). A photograph of CT26 subcutaneous tumors in nude mice and a graph of tumor growth are shown. ( B ) Knockdown of Ccl20 and Cxcl3 inhibits the growth of CT26 subcutaneous tumors in BALB/c mice ( n = 5). A photograph of CT26 subcutaneous tumors in BALB/c mice and a graph of tumor growth are shown. ( C ) Neutralizing antibodies of Ccl20 and Cxcl3 inhibited subcutaneous tumors in BALB/c mice( n = 6). A photograph of CT26 subcutaneous tumors in BALB/c mice and a graph of tumor growth are shown. ( D ) Knockdown of Ccl20 and Cxcl3 inhibits the progression of CT26 orthotopic implanted tumor in BALB/c mice ( n = 5). Representative tumor images and tumor load are shown. ( E ) The percentage of PMN-MDSCs (CD11b + Ly6G + Ly6C low ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. A bar chart indicating statistical values is presented ( n = 5). ( F ) The percentage of Tregs (CD4 + Foxp3 + ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. A bar chart indicating statistical values is presented ( n = 5). ( G and H ) Tumor-infiltrating CD8 + T cells and their granule production (GzmB + ) in TILs (CD45 + ) of orthotopic implanted CRC mice detected by flow cytometry sorting. Bar charts indicating statistical values are presented ( n = 5). ( I ) MDSCs (Gr-1 + ), Tregs (CD4 + , Foxp3 + ), and CD8 + T-cell infiltration in tumor tissues of orthotopic implanted CRC mice. Representative IHC images are shown. ( J ) Histogram showing the number of Gr-1 + , CD4 + , Foxp3 + , and CD8 + cells per ×20 objective lens visual field ( n = 5). Data represent the mean ± SD of 3 independent experiments. We used 2-way ANOVA to determine statistical significance of subcutaneous tumor volume. The remaining statistical methods were conducted using 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005. sh, short hairpin.

Article Snippet: The sections were incubated overnight using primary antibodies CD8 (rabbit; Abcam, ab209775; 1:1,000), CD4 (rabbit; Bioss, bs-0647R; 1:1,000), FOXP3 (rabbit; eBioscience, 14-4771-80; 1:300), Gr-1 (rabbit; BioLegend, 108401; 1:300), CXCL3 (rabbit; Immunoway, YT2075; 1:1,000), CD8 (rabbit; Zsbio, ZA-0508), CD33 (rabbit; Abcam, ab269456), and CD11b (mouse; ORIGENE, TA807952; 1:500) at 4°C.

Techniques: Knockdown, Flow Cytometry, Comparison

( A ) Tumors in the intestines of Apc min/+ tumorigenesis mice ( n = 5). ( B ). Bar chart of tumor load in intestines derived from Apc min/+ mice treated with PD-1 immunotherapy or IgG, with or without 4-HPA (1 mM) ( n = 5). ( C ) Representative images of intestines tumorigenesis visualized by H&E staining. ( D ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) ( n = 5). ( E and F ) Detection of 4-HPA in tumor tissues of patients with CRC by HRGC-MS ( n = 12). ( G – K ) Expression of CD8, CD33, CD11b, and CXCL3 in CRC tissues of patients with CRC analyzed by multiple IF. Visualization of 3 representative cases is shown. Scale bar: 50 μm. Multiple IF detection was performed on the tumor tissues of patients with CRC ( G ). Bar charts of CD8 + T cells ( H ), CD11b + cells ( I ), CD33 + cells ( J ), and MDSCs (CD11b + CD33 + ) ( K ) in CRC tissues ( n = 5). Data represent the mean ± SD of 3 independent experiments. We used 2-way ANOVA to determine the significance of tumor volume of PD-1 treated mice and H&E staining. The remaining statistical analyses were conducted with Student’s t test (2-comparison test) and 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005. rel., relative.

Journal: The Journal of Clinical Investigation

Article Title: Gut microbial metabolite 4-hydroxybenzeneacetic acid drives colorectal cancer progression via accumulation of immunosuppressive PMN-MDSCs

doi: 10.1172/JCI181243

Figure Lengend Snippet: ( A ) Tumors in the intestines of Apc min/+ tumorigenesis mice ( n = 5). ( B ). Bar chart of tumor load in intestines derived from Apc min/+ mice treated with PD-1 immunotherapy or IgG, with or without 4-HPA (1 mM) ( n = 5). ( C ) Representative images of intestines tumorigenesis visualized by H&E staining. ( D ) H&E scoring of tumor-related lesions (including inflammation, adenoma, atypical hyperplasia, and crypt fusion) ( n = 5). ( E and F ) Detection of 4-HPA in tumor tissues of patients with CRC by HRGC-MS ( n = 12). ( G – K ) Expression of CD8, CD33, CD11b, and CXCL3 in CRC tissues of patients with CRC analyzed by multiple IF. Visualization of 3 representative cases is shown. Scale bar: 50 μm. Multiple IF detection was performed on the tumor tissues of patients with CRC ( G ). Bar charts of CD8 + T cells ( H ), CD11b + cells ( I ), CD33 + cells ( J ), and MDSCs (CD11b + CD33 + ) ( K ) in CRC tissues ( n = 5). Data represent the mean ± SD of 3 independent experiments. We used 2-way ANOVA to determine the significance of tumor volume of PD-1 treated mice and H&E staining. The remaining statistical analyses were conducted with Student’s t test (2-comparison test) and 1-way ANOVA with Dunnett’s T3 correct multiple-comparison test. * P < 0.05, ** P < 0.005, *** P < 0.0005. rel., relative.

Article Snippet: The sections were incubated overnight using primary antibodies CD8 (rabbit; Abcam, ab209775; 1:1,000), CD4 (rabbit; Bioss, bs-0647R; 1:1,000), FOXP3 (rabbit; eBioscience, 14-4771-80; 1:300), Gr-1 (rabbit; BioLegend, 108401; 1:300), CXCL3 (rabbit; Immunoway, YT2075; 1:1,000), CD8 (rabbit; Zsbio, ZA-0508), CD33 (rabbit; Abcam, ab269456), and CD11b (mouse; ORIGENE, TA807952; 1:500) at 4°C.

Techniques: Derivative Assay, Staining, Expressing, Comparison